O-304 (ATX-304) AMPK Activator Research Standard

ATX-304 (also called O-304) is a synthetic research compound studied as a pan-AMPK activator. Its reported identity is CAS 1261289-04-6 with a 1,2,4-thiadiazol-3(2H)-one scaffold, not a benzothiazole. Published work describes protection of AMPK phosphorylation from PP2C-mediated dephosphorylation, with activity dependent on existing upstream kinase tone. This page is for laboratory research use only.

Technical Specifications

Mechanism of Action & Research Context

Published work describes ATX-304 as protecting p-T172 AMPK from PP2C-mediated dephosphorylation rather than acting as an AdAM-site allosteric ligand. The reported effect depends on upstream kinase activity, so the experimental model determines whether activation can be observed.

• Enzyme Interaction: Published work describes O-304 as protecting AMPK phosphorylation from PP2C-mediated dephosphorylation. In in vitro models, the observed effect depends on existing upstream kinase activity.

• AMPK Activation: By modulating this pathway, the compound allows researchers to study the downstream phosphorylation of metabolic targets and the regulation of lipid and glucose oxidation in isolated cellular environments.

• Binding Kinetics: The chlorine-substituted 1,2,4-thiadiazol-3(2H)-one scaffold provides structural context for comparing O-304 with other AMPK activators in assay and mechanism studies.

Research Applications

O-304 is utilized as a specialized benchmark for high-fidelity studies in metabolic signaling and enzyme kinetics.

Primary fields of in vitro laboratory investigation include:

• Enzyme Binding Assays: Measuring the binding strength between O-304 and AMPK in cell-free enzyme preparations, often utilizing competitive displacement techniques.

• Kinase Assays: Utilizing fluorescence-based probes to observe how O-304 influences the catalytic efficiency of the AMPK complex.

• Competitive Displacement Studies: Using labeled probes to compete for the same binding pocket, helping to delineate the specificity of the benzothiazole core.

• Structural Biology: Investigating the interaction patterns of chlorine-substituted heterocycles within kinase catalytic sites.

Storage & Handling Guidelines

Store at controlled room temperature. Keep tightly closed.

Storage guidance is a house recommendation. Analytical documentation is per-lot release testing.