SLU-PP-332 (ERR Pan-Agonist) Research Standard

SLU-PP-332 is a potent, synthetic small-molecule pan-agonist of the Estrogen-Related Receptors (ERRs), specifically targeting ERR-alpha, ERR-beta, and ERR-gamma. Built on a hydrazone scaffold that links a 4-hydroxybenzohydrazide core to a naphthalene ring system, this compound is a vital tool for studying mitochondrial metabolism and the transcriptional programs associated with oxidative energy expenditure. Often cited in literature as an “exercise mimetic,” SLU-PP-332 is employed to isolate and investigate the PGC-1-alpha-linked gene programs that drive mitochondrial biogenesis and fatty-acid oxidation at the nuclear-receptor level.

Technical Specifications

Mechanism of Action & Research Context

SLU-PP-332 provides a direct mechanism to modulate the ERR-driven transcriptional machinery, distinguishing its mode of action from upstream energy sensors.

• ERR Isoform Agonism: The compound stabilizes the active conformation of all three ERR isoforms, with the highest relative potency at ERR-alpha (EC50 ≈ 98 nM).

• Transcriptional Regulation: By promoting the recruitment of coactivators to the ERR binding sites, SLU-PP-332 triggers the upregulation of genes involved in mitochondrial biogenesis and fatty-acid oxidation.

• Direct Nuclear Receptor Modulation: Unlike adrenergic agonists or AMPK activators, which modulate mitochondrial activity through indirect signaling cascades, SLU-PP-332 acts directly at the nuclear receptor level to program the metabolic phenotype.

• Selectivity: The compound demonstrates high selectivity for ERRs over classical Estrogen Receptors (ER-alpha/ER-beta) and other related nuclear receptors, making it a reliable tool for isolating ERR-specific biological effects.

Research Applications

SLU-PP-332 is a specialized reagent essential for modern metabolic and biochemical research.

Primary fields of in vitro laboratory investigation include:

• Mitochondrial Biogenesis Research: Studying the molecular pathways that increase mitochondrial density and oxidative capacity.

• Metabolic Signaling: Modeling the transcriptional adaptations of endurance exercise by upregulating PGC-1-alpha-linked gene programs.

• Fatty-Acid Oxidation Studies: Investigating the enzymatic and transcriptional shifts in lipid metabolism within isolated cell lines.

• Nuclear Receptor Profiling: Utilizing the compound to map the interactome of ERR isoforms and their downstream targets in oxidative tissue.

Storage & Handling Guidelines

Store at controlled room temperature. Keep tightly closed. Liquid formats: do not refrigerate or freeze. Cold storage may cause the solution to cloud or precipitate. If this occurs, return to room temperature and mix until clear.

Storage guidance is a house recommendation. Analytical documentation is per-lot release testing.